Sumary of Scientists explore the creation of artificial organelles:
- These compartments have multiple enzymes that work together to execute important cellular functions.
- This was achieved through reprogramming of ‘exosomes’, which are small vesicles (diameter ~120 nm) that cells use for intercellular signaling.
- This allows them to produce high-value biomolecules by performing desired biocatalytic transformations in a spatially confined manner which is not possible using conventional laboratory test tubes.
- The team demonstrated this multienzyme biocatalytic cascade function by encapsulating glucose oxidase (GOx) and horseradish peroxidase (HRP) inside the exosomes.
- The cells derived from human breast tissues were fed with fused exosome nanoreactors, and their internalization over the next 48 hours was observed using various markers and a confocal laser scanning microscope (CLSM).
- Armed with this knowledge, the team sought to create functional artificial mitochondria that are capable of producing energy inside the cells.