Interactions between SARS-CoV-2 S protein and ACE2 receptor using fluorescence correlation spectrosc …

interactions between sars cov 2 s protein and ace2 receptor using fluorescence correlation spectroscopy

Sumary of Interactions between SARS-CoV-2 S protein and ACE2 receptor using fluorescence correlation spectroscopy:

  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is initiated by an interaction between the human angiotensin-converting enzyme type 2 (ACE2) receptor and the SARS-CoV-2 spike (S) protein.
  • Study: Interaction between Spike Protein of SARS-CoV-2 and Human Virus Receptor ACE2 Using Two-Color Fluorescence Cross-Correlation Spectroscopy.
  • The S proteins are composed of two subunits S1 and S2, but only the S1 subunit was used for inhibitor screening in this study.
  • Murine neuroblastoma Neuro2a (N2a) cells were selected as a cell line for SARS-CoV-associated protein expression as these cells display high protein expression due to their high plasmid DNA transfection efficiency.
  • The ACE2, S1, and S2 proteins were purified and concentrated from the lysates.
  • The hACE2, S1, and S1-2 subunits were tagged with fluorescent proteins in N2a cells and observed inside the subcellular compartments, including the endoplasmic reticulum (ER).
  • The results indicate that FCCS quantitatively determines the interaction between the spike protein of SARS-CoV-2 and the human receptor ACE2 in a solution independent of solid-liquid phase-based assays.
  • It is worth noting that the interaction of S protein of SARS-CoV-2 has a higher dissociation constant (Kd) than SARS-CoV, as indicated by surface plasmon resonance (SPR) analysis.

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